Construct: shRNA TRCN0000105261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008992.1-609s1c1
DNA Barcode:
CCTGTGAGCATCTTTGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcd4 (19300)
Gene Description:
ATP-binding cassette, sub-family D (ALD), member 4
Transcript:
RefSeq NM_008992.1 (NON-CURRENT)
Match location:
Position 609 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGAGCATCTTTGGATAT
Hairpin Sequence:
5'-CCGG-CCTGTGAGCATCTTTGGATAT-CTCGAG-ATATCCAAAGATGCTCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGAGCATCTTTGGATATCTCGAGATATCCAAAGATGCTCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGAGCATCTTTGGATATCTCGAGATATCCAAAGATGCTCACAGG-3'

Other clones with same target sequence:

(none)