Construct: shRNA TRCN0000105365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027324.2-2320s1c1
DNA Barcode:
CCAGTCCATGTGTAGCTCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfxn1 (14057)
Gene Description:
sideroflexin 1
Transcript:
RefSeq NM_027324.2 (NON-CURRENT)
Match location:
Position 2320 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCCATGTGTAGCTCTAA
Hairpin Sequence:
5'-CCGG-CCAGTCCATGTGTAGCTCTAA-CTCGAG-TTAGAGCTACACATGGACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCCATGTGTAGCTCTAACTCGAGTTAGAGCTACACATGGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCCATGTGTAGCTCTAACTCGAGTTAGAGCTACACATGGACTGG-3'

Other clones with same target sequence:

  1. TRCN0000317344