Construct: shRNA TRCN0000105371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021517.1-367s1c1
DNA Barcode:
GCCGCTCTGAATGATAAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdzk1 (59020)
Gene Description:
PDZ domain containing 1
Transcript:
RefSeq NM_021517.1 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGCTCTGAATGATAAGAAA
Hairpin Sequence:
5'-CCGG-GCCGCTCTGAATGATAAGAAA-CTCGAG-TTTCTTATCATTCAGAGCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGCTCTGAATGATAAGAAACTCGAGTTTCTTATCATTCAGAGCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGCTCTGAATGATAAGAAACTCGAGTTTCTTATCATTCAGAGCGGC-3'

Other clones with same target sequence:

(none)