Construct: shRNA TRCN0000105429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022813.2-405s1c1
DNA Barcode:
GCAGAACACTGCAGCGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scamp2 (24044)
Gene Description:
secretory carrier membrane protein 2
Transcript:
RefSeq NM_022813.2 (NON-CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAACACTGCAGCGAATTT
Hairpin Sequence:
5'-CCGG-GCAGAACACTGCAGCGAATTT-CTCGAG-AAATTCGCTGCAGTGTTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAACACTGCAGCGAATTTCTCGAGAAATTCGCTGCAGTGTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAACACTGCAGCGAATTTCTCGAGAAATTCGCTGCAGTGTTCTGC-3'

Other clones with same target sequence:

(none)