Construct: shRNA TRCN0000105444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028816.2-1596s1c1
DNA Barcode:
CGATGAGAGTTACATTGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xpo6 (74204)
Gene Description:
exportin 6
Transcript:
RefSeq NM_028816.2 (CURRENT)
Match location:
Position 1596 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGAGAGTTACATTGAGAA
Hairpin Sequence:
5'-CCGG-CGATGAGAGTTACATTGAGAA-CTCGAG-TTCTCAATGTAACTCTCATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGAGAGTTACATTGAGAACTCGAGTTCTCAATGTAACTCTCATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGAGAGTTACATTGAGAACTCGAGTTCTCAATGTAACTCTCATCG-3'

Other clones with same target sequence:

  1. TRCN0000324565