Construct: shRNA TRCN0000105564

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053197.2-1079s1c1
DNA Barcode:
CCTCAGAGAAGCTCCATACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sfxn3 (94280)
Gene Description:
sideroflexin 3
Transcript:
RefSeq NM_053197.2 (NON-CURRENT)
Match location:
Position 1079 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105564)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGAGAAGCTCCATACAT
Hairpin Sequence:
5'-CCGG-CCTCAGAGAAGCTCCATACAT-CTCGAG-ATGTATGGAGCTTCTCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGAGAAGCTCCATACATCTCGAGATGTATGGAGCTTCTCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGAGAAGCTCCATACATCTCGAGATGTATGGAGCTTCTCTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000353994