Construct: shRNA TRCN0000105652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172420.2-435s1c1
DNA Barcode:
CCTTTATTCCAGAGTCAGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r1c (75276)
Gene Description:
protein phosphatase 1, regulatory (inhibitor) subunit 1C
Transcript:
RefSeq NM_172420.2 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTATTCCAGAGTCAGATT
Hairpin Sequence:
5'-CCGG-CCTTTATTCCAGAGTCAGATT-CTCGAG-AATCTGACTCTGGAATAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTATTCCAGAGTCAGATTCTCGAGAATCTGACTCTGGAATAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTATTCCAGAGTCAGATTCTCGAGAATCTGACTCTGGAATAAAGG-3'

Other clones with same target sequence:

(none)