Construct: shRNA TRCN0000105682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_359260.1-1900s1c1
DNA Barcode:
GCACCGTATTTGAGGCCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syde1 (71709)
Gene Description:
synapse defective 1, Rho GTPase, homolog 1 (C. elegans)
Transcript:
RefSeq XM_359260.1 (NON-CURRENT)
Match location:
Position 1900 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCGTATTTGAGGCCCAAA
Hairpin Sequence:
5'-CCGG-GCACCGTATTTGAGGCCCAAA-CTCGAG-TTTGGGCCTCAAATACGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCGTATTTGAGGCCCAAACTCGAGTTTGGGCCTCAAATACGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCGTATTTGAGGCCCAAACTCGAGTTTGGGCCTCAAATACGGTGC-3'

Other clones with same target sequence:

(none)