Construct: shRNA TRCN0000105755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011893.2-2145s1c1
DNA Barcode:
GCCTCTCAATAAATCAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3bp2 (24055)
Gene Description:
SH3-domain binding protein 2
Transcript:
RefSeq NM_011893.2 (NON-CURRENT)
Match location:
Position 2145 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTCAATAAATCAGAGAT
Hairpin Sequence:
5'-CCGG-GCCTCTCAATAAATCAGAGAT-CTCGAG-ATCTCTGATTTATTGAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTCAATAAATCAGAGATCTCGAGATCTCTGATTTATTGAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTCAATAAATCAGAGATCTCGAGATCTCTGATTTATTGAGAGGC-3'

Other clones with same target sequence:

(none)