Construct: shRNA TRCN0000105793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145544.1-135s1c1
DNA Barcode:
CTCAGAATAATGCAGAAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rap1gds1 (229877)
Gene Description:
RAP1, GTP-GDP dissociation stimulator 1
Transcript:
RefSeq NM_145544.1 (NON-CURRENT)
Match location:
Position 135 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGAATAATGCAGAAACAA
Hairpin Sequence:
5'-CCGG-CTCAGAATAATGCAGAAACAA-CTCGAG-TTGTTTCTGCATTATTCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGAATAATGCAGAAACAACTCGAGTTGTTTCTGCATTATTCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGAATAATGCAGAAACAACTCGAGTTGTTTCTGCATTATTCTGAG-3'

Other clones with same target sequence:

(none)