Construct: shRNA TRCN0000105888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007598.2-613s1c1
DNA Barcode:
GCAGGCTTACATCAAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cap1 (12331)
Gene Description:
CAP, adenylate cyclase-associated protein 1 (yeast)
Transcript:
RefSeq NM_007598.2 (NON-CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCTTACATCAAGGAGTT
Hairpin Sequence:
5'-CCGG-GCAGGCTTACATCAAGGAGTT-CTCGAG-AACTCCTTGATGTAAGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCTTACATCAAGGAGTTCTCGAGAACTCCTTGATGTAAGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCTTACATCAAGGAGTTCTCGAGAACTCCTTGATGTAAGCCTGC-3'

Other clones with same target sequence:

(none)