Construct: shRNA TRCN0000105943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172277.1-981s1c1
DNA Barcode:
GATTTGTTGCAGTCCTACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx8 (231834)
Gene Description:
sorting nexin 8
Transcript:
RefSeq NM_172277.1 (NON-CURRENT)
Match location:
Position 981 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTTGTTGCAGTCCTACAAA
Hairpin Sequence:
5'-CCGG-GATTTGTTGCAGTCCTACAAA-CTCGAG-TTTGTAGGACTGCAACAAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTTGTTGCAGTCCTACAAACTCGAGTTTGTAGGACTGCAACAAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTTGTTGCAGTCCTACAAACTCGAGTTTGTAGGACTGCAACAAATC-3'

Other clones with same target sequence:

(none)