Construct: shRNA TRCN0000105951

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153412.1-302s1c1
DNA Barcode:
GCCCGAGGAAATACTCATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phldb2 (208177)
Gene Description:
pleckstrin homology like domain, family B, member 2
Transcript:
RefSeq NM_153412.1 (NON-CURRENT)
Match location:
Position 302 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000105951)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGAGGAAATACTCATCAA
Hairpin Sequence:
5'-CCGG-GCCCGAGGAAATACTCATCAA-CTCGAG-TTGATGAGTATTTCCTCGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGAGGAAATACTCATCAACTCGAGTTGATGAGTATTTCCTCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGAGGAAATACTCATCAACTCGAGTTGATGAGTATTTCCTCGGGC-3'

Other clones with same target sequence:

(none)