Construct: shRNA TRCN0000106001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153537.3-682s1c1
DNA Barcode:
GCAGGAAATCATGGATTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phldb1 (102693)
Gene Description:
pleckstrin homology like domain, family B, member 1
Transcript:
RefSeq NM_153537.3 (NON-CURRENT)
Match location:
Position 682 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAAATCATGGATTCATT
Hairpin Sequence:
5'-CCGG-GCAGGAAATCATGGATTCATT-CTCGAG-AATGAATCCATGATTTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAAATCATGGATTCATTCTCGAGAATGAATCCATGATTTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAAATCATGGATTCATTCTCGAGAATGAATCCATGATTTCCTGC-3'

Other clones with same target sequence:

(none)