Construct: shRNA TRCN0000106026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178119.2-1865s1c1
DNA Barcode:
GCCAAGGACATGAGCAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agap1 (347722)
Gene Description:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Transcript:
RefSeq NM_178119.2 (NON-CURRENT)
Match location:
Position 1865 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGGACATGAGCAGTTTA
Hairpin Sequence:
5'-CCGG-GCCAAGGACATGAGCAGTTTA-CTCGAG-TAAACTGCTCATGTCCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGGACATGAGCAGTTTACTCGAGTAAACTGCTCATGTCCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGGACATGAGCAGTTTACTCGAGTAAACTGCTCATGTCCTTGGC-3'

Other clones with same target sequence:

(none)