Construct: shRNA TRCN0000106030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080448.2-3782s1c1
DNA Barcode:
CCACTCTTACTTGGTGGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Srgap3 (259302)
Gene Description:
SLIT-ROBO Rho GTPase activating protein 3
Transcript:
RefSeq NM_080448.2 (NON-CURRENT)
Match location:
Position 3782 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCTTACTTGGTGGGATT
Hairpin Sequence:
5'-CCGG-CCACTCTTACTTGGTGGGATT-CTCGAG-AATCCCACCAAGTAAGAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCTTACTTGGTGGGATTCTCGAGAATCCCACCAAGTAAGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCTTACTTGGTGGGATTCTCGAGAATCCCACCAAGTAAGAGTGG-3'

Other clones with same target sequence:

(none)