Construct: shRNA TRCN0000106051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013781.2-2575s1c1
DNA Barcode:
CGCTATGAGAAGTTTGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2d3c (27387)
Gene Description:
SH2 domain containing 3C
Transcript:
RefSeq NM_013781.2 (NON-CURRENT)
Match location:
Position 2575 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATGAGAAGTTTGACAAA
Hairpin Sequence:
5'-CCGG-CGCTATGAGAAGTTTGACAAA-CTCGAG-TTTGTCAAACTTCTCATAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATGAGAAGTTTGACAAACTCGAGTTTGTCAAACTTCTCATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATGAGAAGTTTGACAAACTCGAGTTTGTCAAACTTCTCATAGCG-3'

Other clones with same target sequence:

(none)