Construct: shRNA TRCN0000106101

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358819.1-1350s1c1
DNA Barcode:
GCTTATGTGTGAATTGGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acap2 (78618)
Gene Description:
ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Transcript:
RefSeq XM_358819.1 (NON-CURRENT)
Match location:
Position 1350 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106101)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTATGTGTGAATTGGGAAA
Hairpin Sequence:
5'-CCGG-GCTTATGTGTGAATTGGGAAA-CTCGAG-TTTCCCAATTCACACATAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTATGTGTGAATTGGGAAACTCGAGTTTCCCAATTCACACATAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTATGTGTGAATTGGGAAACTCGAGTTTCCCAATTCACACATAAGC-3'

Other clones with same target sequence:

(none)