Construct: shRNA TRCN0000106129

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207223.1-772s1c1
DNA Barcode:
CCTACAGGAGATGGTCAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Acap3 (140500)
Gene Description:
ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
Transcript:
RefSeq NM_207223.1 (CURRENT)
Match location:
Position 772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106129)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACAGGAGATGGTCAACTA
Hairpin Sequence:
5'-CCGG-CCTACAGGAGATGGTCAACTA-CTCGAG-TAGTTGACCATCTCCTGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACAGGAGATGGTCAACTACTCGAGTAGTTGACCATCTCCTGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACAGGAGATGGTCAACTACTCGAGTAGTTGACCATCTCCTGTAGG-3'

Other clones with same target sequence:

(none)