Construct: shRNA TRCN0000106135
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_139206.1-4833s1c1
- DNA Barcode:
- CTGTACTATGAAGTCACTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Arap3 (106952)
- Gene Description:
- ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
- Transcript:
- RefSeq NM_139206.1 (NON-CURRENT)
- Match location:
- Position 4833 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000106135)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGTACTATGAAGTCACTAAA
- Hairpin Sequence:
-
5'-CCGG-CTGTACTATGAAGTCACTAAA-CTCGAG-TTTAGTGACTTCATAGTACAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGTACTATGAAGTCACTAAACTCGAGTTTAGTGACTTCATAGTACAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGTACTATGAAGTCACTAAACTCGAGTTTAGTGACTTCATAGTACAG-3'
Other clones with same target sequence:
-
TRCN0000288345
