Construct: shRNA TRCN0000106161

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133796.3-858s1c1
DNA Barcode:
GAGTGGAATCTCACCATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arhgdia (192662)
Gene Description:
Rho GDP dissociation inhibitor (GDI) alpha
Transcript:
RefSeq NM_133796.3 (NON-CURRENT)
Match location:
Position 858 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106161)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGGAATCTCACCATCAAA
Hairpin Sequence:
5'-CCGG-GAGTGGAATCTCACCATCAAA-CTCGAG-TTTGATGGTGAGATTCCACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGGAATCTCACCATCAAACTCGAGTTTGATGGTGAGATTCCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGGAATCTCACCATCAAACTCGAGTTTGATGGTGAGATTCCACTC-3'

Other clones with same target sequence:

  1. TRCN0000316502