Construct: shRNA TRCN0000106171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139153.1-403s1c1
DNA Barcode:
CCAGAGCTTAAAGTGGGCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Agap3 (213990)
Gene Description:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
Transcript:
RefSeq NM_139153.1 (NON-CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGCTTAAAGTGGGCATA
Hairpin Sequence:
5'-CCGG-CCAGAGCTTAAAGTGGGCATA-CTCGAG-TATGCCCACTTTAAGCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGCTTAAAGTGGGCATACTCGAGTATGCCCACTTTAAGCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGCTTAAAGTGGGCATACTCGAGTATGCCCACTTTAAGCTCTGG-3'

Other clones with same target sequence:

(none)