Construct: shRNA TRCN0000106225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025768.1-1120s1c1
DNA Barcode:
CAGAGCACACACTTCACCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grtp1 (66790)
Gene Description:
GH regulated TBC protein 1
Transcript:
RefSeq NM_025768.1 (NON-CURRENT)
Match location:
Position 1120 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGCACACACTTCACCAAT
Hairpin Sequence:
5'-CCGG-CAGAGCACACACTTCACCAAT-CTCGAG-ATTGGTGAAGTGTGTGCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGCACACACTTCACCAATCTCGAGATTGGTGAAGTGTGTGCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGCACACACTTCACCAATCTCGAGATTGGTGAAGTGTGTGCTCTG-3'

Other clones with same target sequence:

(none)