Construct: shRNA TRCN0000106228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025768.1-99s1c1
DNA Barcode:
GCGGCTTATGAAGAATTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grtp1 (66790)
Gene Description:
GH regulated TBC protein 1
Transcript:
RefSeq NM_025768.1 (NON-CURRENT)
Match location:
Position 99 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTTATGAAGAATTCTTT
Hairpin Sequence:
5'-CCGG-GCGGCTTATGAAGAATTCTTT-CTCGAG-AAAGAATTCTTCATAAGCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTTATGAAGAATTCTTTCTCGAGAAAGAATTCTTCATAAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTTATGAAGAATTCTTTCTCGAGAAAGAATTCTTCATAAGCCGC-3'

Other clones with same target sequence:

(none)