Construct: shRNA TRCN0000106250

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146258.1-4011s1c1
DNA Barcode:
GCCTCTTATTTCTGATGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stard13 (243362)
Gene Description:
StAR-related lipid transfer (START) domain containing 13
Transcript:
RefSeq NM_146258.1 (NON-CURRENT)
Match location:
Position 4011 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106250)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTATTTCTGATGTTTA
Hairpin Sequence:
5'-CCGG-GCCTCTTATTTCTGATGTTTA-CTCGAG-TAAACATCAGAAATAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTATTTCTGATGTTTACTCGAGTAAACATCAGAAATAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTATTTCTGATGTTTACTCGAGTAAACATCAGAAATAAGAGGC-3'

Other clones with same target sequence:

(none)