Construct: shRNA TRCN0000106250
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_146258.1-4011s1c1
- DNA Barcode:
- GCCTCTTATTTCTGATGTTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Stard13 (243362)
- Gene Description:
- StAR-related lipid transfer (START) domain containing 13
- Transcript:
- RefSeq NM_146258.1 (NON-CURRENT)
- Match location:
- Position 4011 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000106250)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTCTTATTTCTGATGTTTA
- Hairpin Sequence:
-
5'-CCGG-GCCTCTTATTTCTGATGTTTA-CTCGAG-TAAACATCAGAAATAAGAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTCTTATTTCTGATGTTTACTCGAGTAAACATCAGAAATAAGAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTCTTATTTCTGATGTTTACTCGAGTAAACATCAGAAATAAGAGGC-3'
Other clones with same target sequence:
(none)
