Construct: shRNA TRCN0000106296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021360.3-1176s1c1
DNA Barcode:
CGCGTCTTCTACCGGATCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Neurl1a (18011)
Gene Description:
neuralized E3 ubiquitin protein ligase 1A
Transcript:
RefSeq NM_021360.3 (NON-CURRENT)
Match location:
Position 1176 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGTCTTCTACCGGATCAAT
Hairpin Sequence:
5'-CCGG-CGCGTCTTCTACCGGATCAAT-CTCGAG-ATTGATCCGGTAGAAGACGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTCTTCTACCGGATCAATCTCGAGATTGATCCGGTAGAAGACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTCTTCTACCGGATCAATCTCGAGATTGATCCGGTAGAAGACGCG-3'

Other clones with same target sequence:

(none)