Construct: shRNA TRCN0000106323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181549.2-1115s1c1
DNA Barcode:
GAAAGAAGAATGTTCCTGTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec18a (353287)
Gene Description:
C-type lectin domain family 18, member A
Transcript:
RefSeq NM_181549.2 (NON-CURRENT)
Match location:
Position 1115 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGAAGAATGTTCCTGTAT
Hairpin Sequence:
5'-CCGG-GAAAGAAGAATGTTCCTGTAT-CTCGAG-ATACAGGAACATTCTTCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGAAGAATGTTCCTGTATCTCGAGATACAGGAACATTCTTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGAAGAATGTTCCTGTATCTCGAGATACAGGAACATTCTTCTTTC-3'

Other clones with same target sequence:

(none)