Construct: shRNA TRCN0000106347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030176.2-198s1c1
DNA Barcode:
CAGATTCTGGTGGAAGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spata2l (78779)
Gene Description:
spermatogenesis associated 2-like
Transcript:
RefSeq NM_030176.2 (CURRENT)
Match location:
Position 198 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATTCTGGTGGAAGACTTT
Hairpin Sequence:
5'-CCGG-CAGATTCTGGTGGAAGACTTT-CTCGAG-AAAGTCTTCCACCAGAATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATTCTGGTGGAAGACTTTCTCGAGAAAGTCTTCCACCAGAATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATTCTGGTGGAAGACTTTCTCGAGAAAGTCTTCCACCAGAATCTG-3'

Other clones with same target sequence:

(none)