Construct: shRNA TRCN0000106393

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009433.2-863s1c1
DNA Barcode:
CAAGCTGATTGTCAAGGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tspyl1 (22110)
Gene Description:
testis-specific protein, Y-encoded-like 1
Transcript:
RefSeq NM_009433.2 (NON-CURRENT)
Match location:
Position 863 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106393)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCTGATTGTCAAGGAATA
Hairpin Sequence:
5'-CCGG-CAAGCTGATTGTCAAGGAATA-CTCGAG-TATTCCTTGACAATCAGCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCTGATTGTCAAGGAATACTCGAGTATTCCTTGACAATCAGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCTGATTGTCAAGGAATACTCGAGTATTCCTTGACAATCAGCTTG-3'

Other clones with same target sequence:

  1. TRCN0000308634