Construct: shRNA TRCN0000106395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010787.1-658s1c1
DNA Barcode:
GACTAACAACTCTGGTCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mea1 (17256)
Gene Description:
male enhanced antigen 1
Transcript:
RefSeq NM_010787.1 (NON-CURRENT)
Match location:
Position 658 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTAACAACTCTGGTCTTAA
Hairpin Sequence:
5'-CCGG-GACTAACAACTCTGGTCTTAA-CTCGAG-TTAAGACCAGAGTTGTTAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTAACAACTCTGGTCTTAACTCGAGTTAAGACCAGAGTTGTTAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTAACAACTCTGGTCTTAACTCGAGTTAAGACCAGAGTTGTTAGTC-3'

Other clones with same target sequence:

  1. TRCN0000317721