Construct: shRNA TRCN0000106432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023734.2-1388s1c1
DNA Barcode:
CCGACTGGGATTTGAAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pi16 (74116)
Gene Description:
peptidase inhibitor 16
Transcript:
RefSeq NM_023734.2 (NON-CURRENT)
Match location:
Position 1388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTGGGATTTGAAGAATT
Hairpin Sequence:
5'-CCGG-CCGACTGGGATTTGAAGAATT-CTCGAG-AATTCTTCAAATCCCAGTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTGGGATTTGAAGAATTCTCGAGAATTCTTCAAATCCCAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTGGGATTTGAAGAATTCTCGAGAATTCTTCAAATCCCAGTCGG-3'

Other clones with same target sequence:

(none)