Construct: shRNA TRCN0000106492

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183171.1-339s1c1
DNA Barcode:
CGTGAATGAATTTGATGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fez1 (235180)
Gene Description:
fasciculation and elongation protein zeta 1 (zygin I)
Transcript:
RefSeq NM_183171.1 (NON-CURRENT)
Match location:
Position 339 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106492)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAATGAATTTGATGAGAA
Hairpin Sequence:
5'-CCGG-CGTGAATGAATTTGATGAGAA-CTCGAG-TTCTCATCAAATTCATTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAATGAATTTGATGAGAACTCGAGTTCTCATCAAATTCATTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAATGAATTTGATGAGAACTCGAGTTCTCATCAAATTCATTCACG-3'

Other clones with same target sequence:

(none)