Construct: shRNA TRCN0000106562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013928.2-603s1c1
DNA Barcode:
TGCCTCACATAAGCGAATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Schip1 (30953)
Gene Description:
schwannomin interacting protein 1
Transcript:
RefSeq NM_013928.2 (NON-CURRENT)
Match location:
Position 603 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTCACATAAGCGAATGTT
Hairpin Sequence:
5'-CCGG-TGCCTCACATAAGCGAATGTT-CTCGAG-AACATTCGCTTATGTGAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTCACATAAGCGAATGTTCTCGAGAACATTCGCTTATGTGAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTCACATAAGCGAATGTTCTCGAGAACATTCGCTTATGTGAGGCA-3'

Other clones with same target sequence:

  1. TRCN0000327344