Construct: shRNA TRCN0000106577

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015748.1-4468s1c1
DNA Barcode:
CGCAAACTCTACTGTCTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slit1 (20562)
Gene Description:
slit homolog 1 (Drosophila)
Transcript:
RefSeq NM_015748.1 (NON-CURRENT)
Match location:
Position 4468 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106577)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAACTCTACTGTCTACAT
Hairpin Sequence:
5'-CCGG-CGCAAACTCTACTGTCTACAT-CTCGAG-ATGTAGACAGTAGAGTTTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAACTCTACTGTCTACATCTCGAGATGTAGACAGTAGAGTTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAACTCTACTGTCTACATCTCGAGATGTAGACAGTAGAGTTTGCG-3'

Other clones with same target sequence:

(none)