Construct: shRNA TRCN0000106585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019626.2-939s1c1
DNA Barcode:
CCCTAACAATTAACGAAGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbln1 (12404)
Gene Description:
cerebellin 1 precursor protein
Transcript:
RefSeq NM_019626.2 (NON-CURRENT)
Match location:
Position 939 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAACAATTAACGAAGTAA
Hairpin Sequence:
5'-CCGG-CCCTAACAATTAACGAAGTAA-CTCGAG-TTACTTCGTTAATTGTTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAACAATTAACGAAGTAACTCGAGTTACTTCGTTAATTGTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAACAATTAACGAAGTAACTCGAGTTACTTCGTTAATTGTTAGGG-3'

Other clones with same target sequence:

(none)