Construct: shRNA TRCN0000106705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003543.3-47s1c1
DNA Barcode:
CTAAGCGTCATCGCAAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C8 (8365)
Gene Description:
H4 clustered histone 8
Transcript:
RefSeq NM_003543.3 (CURRENT)
Match location:
Position 47 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAAGCGTCATCGCAAGGTTT
Hairpin Sequence:
5'-CCGG-CTAAGCGTCATCGCAAGGTTT-CTCGAG-AAACCTTGCGATGACGCTTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAAGCGTCATCGCAAGGTTTCTCGAGAAACCTTGCGATGACGCTTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAAGCGTCATCGCAAGGTTTCTCGAGAAACCTTGCGATGACGCTTAG-3'

Other clones with same target sequence:

  1. TRCN0000232441