Construct: shRNA TRCN0000106729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003540.3-175s1c1
DNA Barcode:
CTTAAGGTGTTCCTGGAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C6 (8361)
Gene Description:
H4 clustered histone 6
Transcript:
RefSeq NM_003540.3 (CURRENT)
Match location:
Position 175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTAAGGTGTTCCTGGAGAAT
Hairpin Sequence:
5'-CCGG-CTTAAGGTGTTCCTGGAGAAT-CTCGAG-ATTCTCCAGGAACACCTTAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTAAGGTGTTCCTGGAGAATCTCGAGATTCTCCAGGAACACCTTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTAAGGTGTTCCTGGAGAATCTCGAGATTCTCCAGGAACACCTTAAG-3'

Other clones with same target sequence:

(none)