Construct: shRNA TRCN0000106744

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003527.4-146s1c1
DNA Barcode:
GAGTTACTCTATCTACGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2BC17 (8348)
Gene Description:
H2B clustered histone 17
Transcript:
RefSeq NM_003527.4 (CURRENT)
Match location:
Position 146 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106744)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTACTCTATCTACGTGTA
Hairpin Sequence:
5'-CCGG-GAGTTACTCTATCTACGTGTA-CTCGAG-TACACGTAGATAGAGTAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTACTCTATCTACGTGTACTCGAGTACACGTAGATAGAGTAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTACTCTATCTACGTGTACTCGAGTACACGTAGATAGAGTAACTC-3'

Other clones with same target sequence:

(none)