Construct: shRNA TRCN0000106758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021059.2-354s1c1
DNA Barcode:
AGACACGAACCTGTGCGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C14 (126961)
Gene Description:
H3 clustered histone 14
Transcript:
RefSeq NM_021059.2 (CURRENT)
Match location:
Position 354 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACACGAACCTGTGCGCCAT
Hairpin Sequence:
5'-CCGG-AGACACGAACCTGTGCGCCAT-CTCGAG-ATGGCGCACAGGTTCGTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACACGAACCTGTGCGCCATCTCGAGATGGCGCACAGGTTCGTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACACGAACCTGTGCGCCATCTCGAGATGGCGCACAGGTTCGTGTCT-3'

Other clones with same target sequence:

(none)