Construct: shRNA TRCN0000106848

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003517.2-267s1c1
DNA Barcode:
CAACGACGAGGAACTGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC20 (8338)
Gene Description:
H2A clustered histone 20
Transcript:
RefSeq NM_003517.2 (CURRENT)
Match location:
Position 267 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106848)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGACGAGGAACTGAACAA
Hairpin Sequence:
5'-CCGG-CAACGACGAGGAACTGAACAA-CTCGAG-TTGTTCAGTTCCTCGTCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGACGAGGAACTGAACAACTCGAGTTGTTCAGTTCCTCGTCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGACGAGGAACTGAACAACTCGAGTTGTTCAGTTCCTCGTCGTTG-3'

Other clones with same target sequence:

(none)