Construct: shRNA TRCN0000106855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170610.2-55s1c1
DNA Barcode:
GCTGTCGTTAAGACCCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2BC1 (255626)
Gene Description:
H2B clustered histone 1
Transcript:
RefSeq NM_170610.2 (CURRENT)
Match location:
Position 55 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTCGTTAAGACCCAGAAA
Hairpin Sequence:
5'-CCGG-GCTGTCGTTAAGACCCAGAAA-CTCGAG-TTTCTGGGTCTTAACGACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTCGTTAAGACCCAGAAACTCGAGTTTCTGGGTCTTAACGACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTCGTTAAGACCCAGAAACTCGAGTTTCTGGGTCTTAACGACAGC-3'

Other clones with same target sequence:

(none)