Construct: shRNA TRCN0000106938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021968.3-177s1c1
DNA Barcode:
GAAGGTGTTCCTGGAGAACGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H4C11 (8363)
Gene Description:
H4 clustered histone 11
Transcript:
RefSeq NM_021968.3 (CURRENT)
Match location:
Position 177 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTGTTCCTGGAGAACGT
Hairpin Sequence:
5'-CCGG-GAAGGTGTTCCTGGAGAACGT-CTCGAG-ACGTTCTCCAGGAACACCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTGTTCCTGGAGAACGTCTCGAGACGTTCTCCAGGAACACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTGTTCCTGGAGAACGTCTCGAGACGTTCTCCAGGAACACCTTC-3'

Other clones with same target sequence:

(none)