Construct: shRNA TRCN0000106969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003531.2-56s1c1
DNA Barcode:
AGCAGCTTGCTACTAAAGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C3 (8352)
Gene Description:
H3 clustered histone 3
Transcript:
RefSeq NM_003531.2 (CURRENT)
Match location:
Position 56 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGCTTGCTACTAAAGCAG
Hairpin Sequence:
5'-CCGG-AGCAGCTTGCTACTAAAGCAG-CTCGAG-CTGCTTTAGTAGCAAGCTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGCTTGCTACTAAAGCAGCTCGAGCTGCTTTAGTAGCAAGCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGCTTGCTACTAAAGCAGCTCGAGCTGCTTTAGTAGCAAGCTGCT-3'

Other clones with same target sequence:

(none)