Construct: shRNA TRCN0000106977

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021064.3-71s1c1
DNA Barcode:
AGGCCAAGACTCGCTCTTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC11 (8969)
Gene Description:
H2A clustered histone 11
Transcript:
RefSeq NM_021064.3 (NON-CURRENT)
Match location:
Position 71 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000106977)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCCAAGACTCGCTCTTCTA
Hairpin Sequence:
5'-CCGG-AGGCCAAGACTCGCTCTTCTA-CTCGAG-TAGAAGAGCGAGTCTTGGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCCAAGACTCGCTCTTCTACTCGAGTAGAAGAGCGAGTCTTGGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCCAAGACTCGCTCTTCTACTCGAGTAGAAGAGCGAGTCTTGGCCT-3'

Other clones with same target sequence:

(none)