Construct: shRNA TRCN0000107006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003521.2-54s1c1
DNA Barcode:
CATTAACAAGGCTCAGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2BC14 (8342)
Gene Description:
H2B clustered histone 14
Transcript:
RefSeq NM_003521.2 (CURRENT)
Match location:
Position 54 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAACAAGGCTCAGAAGAA
Hairpin Sequence:
5'-CCGG-CATTAACAAGGCTCAGAAGAA-CTCGAG-TTCTTCTGAGCCTTGTTAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAACAAGGCTCAGAAGAACTCGAGTTCTTCTGAGCCTTGTTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAACAAGGCTCAGAAGAACTCGAGTTCTTCTGAGCCTTGTTAATG-3'

Other clones with same target sequence:

(none)