Construct: shRNA TRCN0000107021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173674.1-741s1c1
DNA Barcode:
GCAGGAATAATTGCTGATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCBLD1 (285761)
Gene Description:
discoidin, CUB and LCCL domain containing 1
Transcript:
RefSeq NM_173674.1 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAATAATTGCTGATGAA
Hairpin Sequence:
5'-CCGG-GCAGGAATAATTGCTGATGAA-CTCGAG-TTCATCAGCAATTATTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAATAATTGCTGATGAACTCGAGTTCATCAGCAATTATTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAATAATTGCTGATGAACTCGAGTTCATCAGCAATTATTCCTGC-3'

Other clones with same target sequence:

(none)