Construct: shRNA TRCN0000107031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002405.2-315s1c1
DNA Barcode:
CCTAAGCTACAGCTACACGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MFNG (4242)
Gene Description:
MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Transcript:
RefSeq NM_002405.2 (NON-CURRENT)
Match location:
Position 315 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGCTACAGCTACACGAT
Hairpin Sequence:
5'-CCGG-CCTAAGCTACAGCTACACGAT-CTCGAG-ATCGTGTAGCTGTAGCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGCTACAGCTACACGATCTCGAGATCGTGTAGCTGTAGCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGCTACAGCTACACGATCTCGAGATCGTGTAGCTGTAGCTTAGG-3'

Other clones with same target sequence:

(none)