Construct: shRNA TRCN0000107089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006109.2-1400s1c1
DNA Barcode:
CTGGGCTCATTTGCTGACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRMT5 (10419)
Gene Description:
protein arginine methyltransferase 5
Transcript:
RefSeq NM_006109.2 (NON-CURRENT)
Match location:
Position 1400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGGCTCATTTGCTGACAAT
Hairpin Sequence:
5'-CCGG-CTGGGCTCATTTGCTGACAAT-CTCGAG-ATTGTCAGCAAATGAGCCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGGCTCATTTGCTGACAATCTCGAGATTGTCAGCAAATGAGCCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGGCTCATTTGCTGACAATCTCGAGATTGTCAGCAAATGAGCCCAG-3'

Other clones with same target sequence:

(none)