Construct: shRNA TRCN0000107098

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019049.1-689s1c1
DNA Barcode:
CGAATAAATCTAATGCTCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf218 (54530)
Gene Description:
chromosome 1 open reading frame 218
Transcript:
RefSeq NM_019049.1 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107098)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATAAATCTAATGCTCCTA
Hairpin Sequence:
5'-CCGG-CGAATAAATCTAATGCTCCTA-CTCGAG-TAGGAGCATTAGATTTATTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATAAATCTAATGCTCCTACTCGAGTAGGAGCATTAGATTTATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATAAATCTAATGCTCCTACTCGAGTAGGAGCATTAGATTTATTCG-3'

Other clones with same target sequence:

(none)