Construct: shRNA TRCN0000107134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002514.2-387s1c1
DNA Barcode:
AGAGGGAGATAACTGTGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCN3 (4856)
Gene Description:
cellular communication network factor 3
Transcript:
RefSeq NM_002514.2 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000107134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGGAGATAACTGTGTGTT
Hairpin Sequence:
5'-CCGG-AGAGGGAGATAACTGTGTGTT-CTCGAG-AACACACAGTTATCTCCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGGAGATAACTGTGTGTTCTCGAGAACACACAGTTATCTCCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGGAGATAACTGTGTGTTCTCGAGAACACACAGTTATCTCCCTCT-3'

Other clones with same target sequence:

(none)